We had this problem appear completely by random in AutoDesk ADMS Console 2008. We were suddenly being prompted to enter an Impersonation Username and Password for ASP and even though the correct password was entered, it would still error.
This error occurred after the Domain Admin password was changed. There is an option to use an explicit Domain account for the DefaultAppPool in its properties under the identify tab. I changed this to the new password and everything started working again.
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